TECHNICAL NOTE: WORKFLOW FOR RESPIRATORY MULTIPLEX PCR

The need for robust and reliable population testing of SARS-CoV-2 will remain for years to come. As we move into the next phase of this global health crisis, it has become clear that simple and effective lab reagents and kits need to remain readily available to evaluate viral respiratory clinical samples.

The need for a diagnostic test from a single type of sample input, to answer the singular question of COVID positive or negative, has also needed to evolve. Especially as we move into the fall and winter months with higher respiratory infection incidence. The ability to evaluate a clinical sample for a panel of common (and highly infectious) viral respiratory pathogens will become increasingly important.

The Launchworks 2X One-Step RT-qPCR Master Mix, Viral RNA Extraction Kit, and in-house developed primer sets meet this need. Launchworks has developed a RT-PCR workflow that reliably detects across five viral targets: Influenza A, Influenza B, RSV (pan), SARS-CoV-2 E-gene and SARS-CoV-2 N-gene.

This technical note presents the results from an initial study that was done using the three Launchworks products described above: 2X One-Step RT-qPCR Master Mix, Viral RNA Extraction Kit, and in-house developed primer sets. Laboratories that are interested in workflow for viral respiratory pathogen testing should contact Launchworks and validate with their own internal laboratory and operating procedures.

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